Biorepository and Tissue Analytics Shared Resource | MCW Cancer Center | Medical College of Wisconsin Cancer Center

Scheduling, Location, and Hours of Operation

Biorepository: Dynacare Building, lower level
Tissue Analytics: Translational and Biomedical Research Center, 4th floor
Monday–Friday from 8 a.m.-5 p.m.

Submit a BTASR iLab request

Services and Technology

BTASR provides a broad range of biospecimen services, including standard and custom processing, leading-edge digital pathology analytics, clinical trial support, and storage. In addition, BTASR is CAP-accredited and CLIA-compliant, which ensures quality operation, access to meticulously collected and quality-controlled specimens, vetted protocols, and standard operating procedures.

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Tumor Tissues and Biofluids

Frozen/fresh tumor tissue for PDX, ex vivo explants/organoids, cell lines
Viable bone marrow, whole blood, cord blood, plasma, buffy coat

Tissue Microarray Construction

Standard core-based TMAs/matrix assembly arrays
Sausage arrays (pos/neg controls)/Cell pellet arrays

Multiplex Immunofluorescence and Quantitative Histocytometry

Solid tumor immunoprofiling: CD4, CD8, CD20, FoxP3, CD68, pan-CK, DAPI or custom
Quantitative multiplex IF imaging: Leica Bond Rx/Akoya PhenoImager; imaging software (Tissue Studio, Inform, QuPath)

Nucleic Acid (RNA/DNA) Purification

Frozen tissue: Qiagen column purification
FFPE tissue: Purigen Ionic isotachophoresis (sequencing quality)

Storage Services

Data logging and barcoded labeling of samples
Monitored, secure -80°C storage, LN/-150°C cryostorage

Technologies and Equipment

Freezers: 15 -80, 2 -150 and 3 liquid nitrogen dewars
Histology: tissue processor, microtomes, embedding stations, autostainer, and coverslipper
Purigen Ionic: DNA/RNA extraction from FFPE specimens
Tissue arrayer: 3DHistech Grandmaster
Immunostainers: Dako Omnis; Leica Bond Rx
Slide scanners: 3DHistech Pannoramic Flash (bright field and IF) and Akoya PhenoImager (7-9 color IF scanner)