Structural Biology Shared Resource (SBSR)

The Structural Biology Shared Resource enables advanced atomic-level studies on the structure and function of cancer related molecules, macromolecules, and their interactions with drugs and biologicals.

Scheduling, Location, and Hours of Operation

Protein Purification Unit: Translational Biomedical Research Center (TBRC), 3rd floor
Cryo-EM facility: Health Research Center, Basement
Monday–Friday from 8 a.m.-5 p.m.
Submit a SBSR iLab Request

Services and Technologies

The SBSR consists of the Protein Purification unit and the Structure Determination unit. The Protein Purification unit produces homogeneous, high-quality proteins well suited for downstream biophysical studies and structure determination. The Structure Determination unit employs X-ray crystallography, Cryo-EM, and advanced AI-assisted analytical tools for structure determination and imaging.

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Protein Production
  • I26R New Brunswick incubators
  • Aeolus compact CO2 incubators
  • Guava easyCyte cytometer
  • Optima XPN-80
  • NGC Bio-Rad
  • Akta Start
  • Shimatzu HPLC
  • Agilent 1260 Infinity
  • Thermo Ultimate 3000
  • Agilent Cary Eclipse
Cryo-EM
  • PELCO easiGlow
  • Thermo Fisher Glacios
  • Falcon 4 detector
  • Ceta-D Camera
  • Selectris
  • GPU workstations
Structural Biology Shared Resource

iLab Requests and Support

Access the links below to learn how to register for iLab and submit requests.

Leadership

headshot

Wei Liu, PhD

Director, Structural Biology Shared Resource (SBSR)

weiliu@mcw.edu

Notable Publications

CXXC5 drove inflammation and ovarian cancer proliferation via transcriptional activation of ZNF143 and EGR1. (Geng T, Sun Q, He J, Chen Y, Cheng W, Shen J, Liu B, Zhang M, Wang S, Asan K, Song M, Gao Q, Song Y, Liu R, Liu X, Ding Y, Jing A, Ye X, Ren H, Zeng K, Zhou Y, Zhang B, Ma S, Liu W, Liu S, Ji J) Cell Signal 2024 Jul;119:111180. PMID: 38642782; DOI: 10.1016/j.cellsig.2024.111180; Scopus ID: 2-s2.0-85190884720

FBXO5-mediated RNF183 degradation prevents endoplasmic reticulum stress-induced apoptosis and promotes colon cancer progression. (Ji J, Jing A, Ding Y, Ma X, Qian Q, Geng T, Cheng W, Zhang M, Sun Q, Ma S, Wang X, Yuan Q, Xu M, Qin J, Ma L, Yang J, He J, Du Q, Xia M, Xu Y, Chen Z, Zhu L, Liu W, Liu S, Liu B) Cell Death Dis 2024 Jan 11;15(1):33). PMID: 38212299; PMCID: PMC10784456; DOI: 10.1038/s41419-024-06421-2; Scopus ID: 2-s2.0-85182091692

FOXP4-mediated induction of PTK7 activates the Wnt/β-catenin pathway and promotes ovarian cancer development. (Ji J, Qian Q, Cheng W, Ye X, Jing A, Ma S, Ding Y, Ma X, Wang Y, Sun Q, Wang X, Chen Y, Zhu L, Yuan Q, Xu M, Qin J, Ma L, Yang J, Zhang M, Geng T, Wang S, Wang D, Song Y, Zhang B, Xu Y, Xu L, Liu S, Liu W, Liu B) Cell Death Dis 2024 May 13;15(5):332. PMID: 38740744; PMCID: PMC11091054; DOI: 10.1038/s41419-024-06713-7; Scopus ID: 2-s2.0-85192951272 

Structure-Based Lead Optimization of Enterovirus D68 2A Protease Inhibitors. (Tan B, Liu C, Li K, Jadhav P, Lambrinidis G, Zhu L, Olson L, Tan H, Wen Y, Kolocouris A, Liu W, Wang J) J Med Chem 2023 Nov 09;66(21):14544-14563. PMID: 37857371; DOI: 10.1021/acs.jmedchem.3c00995; Scopus ID: 2-s2.0-85176509223

Cryo-EM structure of the human adenosine A2B receptor-Gs signaling complex. (Chen Y, Zhang J, Weng Y, Xu Y, Lu W, Liu W, Liu M, Hua T, Song G) Sci Adv 2022 Dec 23;8(51):eadd3709. PMID: 36563137; PMCID: PMC9788782; DOI: 10.1126/sciadv.add3709; Scopus ID: 2-s2.0-85144637416

An Isochroman Analog of CD3254 and Allyl-, Isochroman-Analogs of NEt-TMN Prove to Be More Potent Retinoid-X-Receptor (RXR) Selective Agonists Than Bexarotene. (Jurutka PW, di Martino O, Reshi S, Mallick S, Sausedo MA, Moen GA, Lee IJ, Ivan DJ, Krall TD, Peoples SJ, Perez A, Tromba L, Le A, Khadka I, Petros R, Savage BM, Salama E, Salama J, Ziller JW, Noh Y, Lee MY, Liu W, Welch JS, Marshall PA, Wagner CE) Int J Mol Sci 2022 Dec 19;23(24). PMID: 36555852; PMCID: PMC9782500; DOI: 10.3390/ijms232416213; Scopus ID: 2-s2.0-85144580245

Structural basis for receptor selectivity and inverse agonism in S1P5 receptors. (Lyapina E, Marin E, Gusach A, Orekhov P, Gerasimov A, Luginina A, Vakhrameev D, Ergasheva M, Kovaleva M, Khusainov G, Khorn P, Shevtsov M, Kovalev K, Bukhdruker S, Okhrimenko I, Popov P, Hu H, Weierstall U, Liu W, Cho Y, Gushchin I, Rogachev A, Bourenkov G, Park S, Park G, Hyun HJ, Park J, Gordeliy V, Borshchevskiy V, Mishin A, Cherezov V) Nat Commun 2022 Aug 12;13(1):4736. PMID: 35961984; PMCID: PMC9374744; DOI: 10.1038/s41467-022-32447-1; Scopus ID: 2-s2.0-85135832975

Structures of β1-adrenergic receptor in complex with Gs and ligands of different efficacies. (Su M, Paknejad N, Zhu L, Wang J, Do HN, Miao Y, Liu W, Hite RK, Huang XY) Nat Commun 2022 Jul 14;13(1):4095. PMID: 35835792; PMCID: PMC9283524; DOI: 10.1038/s41467-022-31823-1; Scopus ID: 2-s2.0-85134147752